The Role of IL-7 in Thymic and Extrathymic Development of TCRγδ Cells
作者： L. Puddington , U. von Freeden-Jeffry , L. Lefrançois , K. Laky , R. Murray
DOI:
关键词:
摘要: IL-7-deficient (IL-7 −/− ) mice have reduced numbers of B and TCRαβ cells, but lack mature TCRγδ cells. Although most T cell development occurs in the thymus, some intestinal intraepithelial lymphocytes (IEL), including can develop extrathymically. Epithelial cells both thymus intestine synthesize IL-7, suggesting that could occur either site. To evaluate role thymic IL-7 newborn TCRβ-deficient (TCRβ thymi were grafted to mice. Donor- host-derived recovered from grafts, spleen, IEL. However, when TCRβ mice, no graft-derived occurred, indicating extrathymic did not support IEL generation precursors. In contrast, occurred efficiently adult, thymectomized, irradiated C57BL/6J reconstituted with bone marrow. This demonstrated required production. Thus, intrathymic was for while peripheral sufficient
参考文章(50)
Günter J. Hämmerling, Ulrich Hämmerling, John F. Kearney, Monoclonal antibodies and T-cell hybridomas : perspectives and technical advances Elsevier/North-Holland Biomedical Press , Sole distributors for the U.S.A. and Canada, Elsevier North-Holland. ,(1981)
A E Namen, S G Reed, R F Voice, K H Grabstein, K Shanebeck, M B Widmer, Regulation of T cell proliferation by IL-7. Journal of Immunology. ,vol. 144, pp. 3015- 3020 ,(1990)
M De Smedt, G Leclercq, J Plum, Exogenous IL-7 promotes the growth of CD3-CD4-CD8-CD44+CD25+/- precursor cells and blocks the differentiation pathway of TCR-alpha beta cells in fetal thymus organ culture. Journal of Immunology. ,vol. 150, pp. 2706- 2716 ,(1993)
V Salomon-Vie, F Heuze, F Jotereau, H Gascan, Cell kinetics in the fetal mouse thymus: precursor cell input, proliferation, and emigration. Journal of Immunology. ,vol. 138, pp. 1026- 1030 ,(1987)
D I Godfrey, A Zlotnik, T Suda, Phenotypic and functional characterization of c-kit expression during intrathymic T cell development. Journal of Immunology. ,vol. 149, pp. 2281- 2285 ,(1992)
L Lefrançois, S Olson, A novel pathway of thymus-directed T lymphocyte maturation. Journal of Immunology. ,vol. 153, pp. 987- 995 ,(1994)
S Okada, H Nakauchi, K Nagayoshi, S Nishikawa, S Nishikawa, Y Miura, T Suda, Enrichment and characterization of murine hematopoietic stem cells that express c-kit molecule Blood. ,vol. 78, pp. 1706- 1712 ,(1991) , 10.1182/BLOOD.V78.7.1706.1706
A L Glasebrook, F W Fitch, M Sarmiento, IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement. Journal of Immunology. ,vol. 125, pp. 2665- 2672 ,(1980)
L Puddington, L Lefrançois, K Laky, Age-dependent intestinal lymphoproliferative disorder due to stem cell factor receptor deficiency: parameters in small and large intestine. Journal of Immunology. ,vol. 158, pp. 1417- 1427 ,(1997)
J Kennedy, D I Godfrey, A Zlotnik, P Mombaerts, S Tonegawa, Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation. Journal of Immunology. ,vol. 152, pp. 4783- 4792 ,(1994)