In vitro synergistic cytotoxicity of gemcitabine and pemetrexed and pharmacogenetic evaluation of response to gemcitabine in bladder cancer patients.

摘要: The present study was performed to investigate the capability of gemcitabine and pemetrexed synergistically interact with respect cytotoxicity apoptosis in T24 J82 bladder cancer cells, establish a correlation between drug activity gene expression selected genes tumour samples. interaction synergistic; indeed, favoured by increasing cellular population S-phase, reducing Akt phosphorylation as well inducing major uptake system, human equilibrative nucleoside transporter-1 (hENT1), key activating enzyme deoxycytidine kinase (dCK) both cell lines. Bladder specimens showed an heterogeneous pattern patients higher levels dCK hENT1 had better response. Moreover, concentrative detectable only 3/12 patients, two whom presented complete response gemcitabine. These data provide evidence that chemotherapeutic combination is synergistic against cells vitro assessment involved activation might be possible determinant may represent new tool for treatment optimization.