Polymorphisms in Pfcrt and Pfmdr-1 genes after five years withdrawal of chloroquine for the treatment of Plasmodium falciparum malaria in West Bengal, India.

摘要: Abstract Background The emergence of resistant power against different antimalarial agents particularly by Plasmodium falciparum is a challenge to combat malaria. Regular monitoring essential not only determine the efficacy and development resistance parasite but also detect early sign regaining sensitivity any anti-malarial agent that has been withdrawn for long period. Studies on molecular markers associated with drug prevailing population play an important role in this aspect. present protocol was designed study polymorphisms pfcrt pfmdr-1 gene chloroquine among P. after five years artemisinin combination therapy (ACT) implementation. Methods Clinical isolates were collected from positive patients attending malaria clinic Calcutta School Tropical Medicine during December 2014 2015. Genomic parasitic DNA extracted subjected sequencing directly purified PCR products. Results A total 89 sequenced 73 genes. In mutant K76 T detected all S VMN haplotype. Out three Y184 F isolates. One synonymous G182 G one non-synonymous S232 F/Y , mutation 99% Conclusion All carrying gene, considered as hall mark CQ resistance, indicate there no area ACT