Evaluation of BRCA1 -related molecular features and microRNAs as prognostic factors for triple negative breast cancers

摘要: The BRCA1 gene plays a key role in triple negative breast cancers (TNBCs), which its expression can be lost by multiple mechanisms: germinal mutation followed deletion of the second allele; regulation promoter methylation; or miRNA-mediated silencing. This study aimed to establish correlation among BRCA1-related molecular parameters, tumor characteristics and clinical follow-up patients find new prognostic factors. protein mRNA was quantified situ TNBCs 69 patients. methylation status checked, as well cytokeratin 5/6 expression. Maintenance expressed interaction with BARD1 quantified, marker functionality, profiles 27 microRNAs were determined. miR-548c-5p emphasized independent factor TNBC. A combination tumoral miR-548c three other known parameters (tumor size, lymph node invasion CK status) allowed for relapse prediction logistic regression an area under curve (AUC) = 0.96. expression, amount ligated tumor, lacked any associations patient outcomes, likely due high intratumoral heterogeneity, thus could not used purposes. In purposes at time diagnosis. contrast, showed promising potential