作者: K. M. Olsen , J. C. Daly
DOI: 10.1603/0022-0493-93.4.1293
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摘要: In Australia, transgenic cotton plants expressing the cry1Ac gene from Bacillus thu- ringiensis Berliner variety kurstaki are less toxic to first-instar Helicoverpa armigera (Hubner) after plant is producing fruit. We developed two bioassay methods (leaf mush, leaf disk) test if physiological state of explained changes in toxicity and a third method (diet incorporation) was quantify Bt leaves when mixed chickpea diet. Cry1Ac protein H. larvae with fruiting versus presquare conventional cotton. Differences LC50 varied 2.4- 726-fold, depending on source toxin material. These results suggest that plant-toxin interactions reducing protein. The possible role tannins these discussed.