Running Title: SOX2 knockdown in bovine embryos Summary sentence: SOX2 is a protein marker for bovine ICM; however, SOX2 knockdown in one blastomere of a two-cell embryo is compatible with preimplantation development and daughter cells can contribute to the ICM.

作者： Marcelo D. Goissis , Jose B. Cibelli

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摘要: To date, efforts to establish pluripotent embryonic stem cells (ESCs) from bovine embryos have failed. The lack of reliable pluripotency markers is an important drawback when attempting derive these cells. This study aimed identify genes upregulated in the inner cell mass (ICM) blastocysts, and we selected SOX2 for further characterization. Spatial temporal localization protein revealed that its expression starts at 16-cell stage then becomes restricted ICMs blastocysts. role during early development embryos, designed siRNA target SOX2. We began by injecting this into zygotes; rate which blastocysts developed declined, compared noninjected or scramble-injected controls. When only one blastomere a twocell embryo was injected with siRNA, observed rates similar those Daughter were tracked TRITC fluorescence found contribute ICM as select also lacked Gene analysis decrease NANOG gene siRNA-injected but OCT4 remained unchanged. conclude localizes

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Running Title: SOX2 knockdown in bovine embryos Summary sentence: SOX2 is a protein marker for bovine ICM; however, SOX2 knockdown in one blastomere of a two-cell embryo is compatible with preimplantation development and daughter cells can contribute to the ICM.

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Running Title: SOX2 knockdown in bovine embryos Summary sentence: SOX2 is a protein marker for bovine ICM; however, SOX2 knockdown in one blastomere of a two-cell embryo is compatible with preimplantation development and daughter cells can contribute to the ICM.

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