Clinical and analytical relevance of NNRTIs minority mutations on viral failure in HIV-1 infected patients.

摘要: The objective of this study was to assess the analytical and clinical relevance minority variants using a new pyrosequencing (PSQ) assay detect minor with frequencies below current 20% setting limit. A PSQ approach for detecting quantifying mutations developed analysis 14 codons human immunodeficiency virus (HIV) reverse transcriptase (RT) gene limit conventional sequencing. Ten patients who experienced virological failure (VF) after first-line regimen lamivudine, tenofovir, either efavirenz, nevirapine, or etravirine, as well 10 controls without VF, were included in retrospective study. Baseline plasma from time VF assessed Sanger sequencing methods. sensitivity detection sequence is 5%. At baseline, no variant detected 10/10 patient both assays, whereas, two failed therapy had baseline non-nucleoside inhibitor (NNRTI) that not by standard genotyping. genotyping four out patients, five patients. Clinically, at 10% level can be efficiently used suitable predictor evolution viral populations. These traits allow better interpretation data analysis, which help clinicians providing treatment HIV. J. Med. Virol. 86:394–403, 2014. © 2013 Wiley Periodicals, Inc.