Comparison of ultra-deep versus Sanger sequencing detection of minority mutations on the HIV-1 drug resistance interpretations after virological failure.

摘要: Objective: Drug-resistance mutations are routinely detected using standard Sanger sequencing, which does not detect minor variants with a frequency below 20%. The impact of detecting generated by ultra-deep sequencing (UDS) on HIV drug-resistance interpretations has yet been studied. Design: Fifty HIV-1 patients who experienced virological failure were included in this retrospective study. Methods: UDS protocol allowed the detection and quantification protease reverse transcriptase related to genotypes A, B, C, F G. DeepChek-HIV simplified interpretation software was used compare UDS. Results: total time required for found be approximately three times longer than equivalent reagent costs. allof themutations foundby population sequencingand identifiedadditional resistance all patients. An analysis drug revealed 643 224 clinically relevant respectively. Three more 20% prevalence solely UDS: A98S (23%), E138A (21%) V179I (25%). A significant difference drugresistance 19 antiretroviral drugs observed between methods. Y181C T215Y most frequent associated differences. Conclusion: combination DeepChek results would help clinicians provide suitable treatments. cut-off 1% better characterization viral identifying additional improving interpretation. 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins AIDS 2014, 28:000‐000