Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae.

摘要: Abstract Restriction enzyme-mediated events (REM events; integration of transforming DNA catalyzed by in vivo action a restriction enzyme) and illegitimate recombination (IR that shares no homology with the host genomic sequences) have been previously characterized Saccharomyces cerevisiae. This study determines effect mutations genes are involved homologous and/or repair double-stranded breaks on these events. Surprisingly, REM completely independent double-strand-break functions encoded RAD51, RAD52, RAD57 but require RAD50 gene product. IR under different genetic control than In rad50 mutant, occurred at wild-type frequency, whereas frequency was 20- to 100-fold reduced. Conversely, rad52 mutant grossly deficient (at least 1,000-fold reduced) showed only 2- 8-fold reduction frequency.