Guinea Pig Lymphotoxin (LT) II. Physicochemical Properties of LT Produced by Lymphocytes Stimulated with Antigen or Concanavalin A: Its Differentiation from Migration-Inhibitory Factor (MIF)

摘要: Guinea pig lymphotoxin (LT) produced by stimulation of sensitized lymph node cells, specifically antigen or non-specifically concanavalin A, was studied methods previously used to characterize guinea migration-inhibitory factor. The results showed that LT induced either mitogen heat labile, eluted from Sephadex columns with peak activity in fractions containing molecules 35,000 55,000 m.w., had an electrophoretic mobility at pH 9.1 similar albumin and a buoyant density on CsCl protein. It destroyed chymotrypsin but not neuraminidase. These studies show can be readily differentiated On the other hand, has many features common A.