Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor.

摘要: MPHOSPH1, which is one of the kinesin superfamily proteins, has been reported to play an essential role in carcinogenesis and progression several kinds cancers. MPHOSPH1 also suggested be involved STAT3 phosphorylation hepatocellular carcinoma. However, biological behavior testicular germ cell tumors (TGCTs) unclear at present. The purposes this study were investigate correlation between expression clinicopathological factors examine efficacy target therapy TGCTs. We investigated 75 formalin-fixed paraffin-embedded TGCT samples, containing a total 86 tumor components, by immunohistochemistry 12 frozen samples Western blotting. Moreover, we carried out vitro studies clarify antitumor effect knockdown embryonal carcinoma lines, NEC8 NEC14, using small interference RNA (siRNA). A significantly high was recognized yolk sac components compared seminoma component (p<0.001, respectively). Clinically, non-seminoma cases are known have worse prognosis than pure-seminoma cases. Interestingly, associated with distant metastasis (p=0.001), thus advanced-stage disease study. High interacted phosphorylated (p=0.01). experiments demonstrated that interruption siRNA resulted significant reduction migration, invasion, proliferation colony formation both lines In conclusion, may potential treatment option for TGCTs, its novel biomarker poor prognosis.