Activation of the Akt-mTOR and MAPK pathways in dedifferentiated liposarcomas.

摘要: The Akt/mTOR and MAPK pathways play important roles in modulating cellular function response to extracellular signals, they are known be activated certain kinds of sarcomas. Few investigations have examined these dedifferentiated liposarcoma (DDLS), relation clinicopathological features. Clinicopathological immunohistochemical analyses were conducted using 99 DDLS specimens. An vitro study was also examine the antitumor effects an mTOR inhibitor a MEK on two cell lines. revealed that AJCC staging significant prognostic factor for overall survival tumor size, depth, location factors event-free survival. Phosphorylated Akt (pAkt), pmTOR, pS6RP, p4E-BP1, pMEK, pERK expressions positive 57.4, 52.4, 71.4, 57.1, 84.1, 50.8 % component 63 primary DDLSs. Positive staining pmTOR significantly more frequent than well-differentiated component. A univariate analysis expression associated with poor prognosis tumors retroperitoneum/ventral body cavity. inhibitors dose-dependently inhibited proliferation both lines decreased downstream pS6RP pERK, respectively. combined use enhanced antiproliferative activity. In conclusion, specimens, inhibition Our findings suggest could therapeutic target patients DDLS.