In Vivo Fluorescence Correlation and Cross-Correlation Spectroscopy
作者： Jörg Mütze , Thomas Ohrt , Zdeněk Petrášek , Petra Schwille
DOI: 10.1007/978-3-642-02597-6_7
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摘要: In this manuscript, we describe the application of Fluorescence Correlation Spectroscopy (FCS), Cross-Correlation (FCCS), and scanning FCS (sFCS) to two in vivo systems. first part, two-photon standard Caenorhabditis elegans embryos. The differentiation a single fertilized egg into complex organism C. is regulated by number protein-dependent processes. oocyte divides asymmetrically daughter cells different developmental fate. Two involved proteins, PAR-2 NMY-2, are studied. second investigated system mechanism RNA interference human cells. An EGFP based cell line that allows study dynamics localization RNA-induced silencing (RISC) with created, which has so far been inaccessible other experimental methods. Furthermore, employed highlight asymmetric incorporation labeled siRNAs RISC.
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