Validation of a modified algorithm for the identification of yeast isolates using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS).
作者： B. H. Herendael , P. Bruynseels , M. Bensaid , T. Boekhout , T. Baere
DOI: 10.1007/S10096-011-1383-Y
关键词:
摘要: Optimising antifungal treatment requires the fast and species-specific identification of yeast isolates. We evaluated a modified protocol for rapid clinical isolates using matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) technology. First, we simplified extraction procedure 54 Second, validated new with this lower threshold by analysing 167 either MALDI-TOF or conventional techniques. analysis both standard short yielded identical results, although log-scores were latter. With protocol, 163/167 (97.6%) showed correct as compared to A total 135 out 163 (82.8%) identifications above 1.7, which considered minimum log-score secure species identification. The is crucial in patient management. technique can be an alternative labourious procedure, will lower. practical accurate If isolate shows below should used.
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