Induction of cellular immune responses by a murine monoclonal anti-idiotypic antibody recognizing the 791Tgp72 antigen expressed on colorectal, gastric and ovarian human tumours.

摘要: There is accumulating evidence that cellular rather than antibody responses are more effective for tumour rejection. It therefore important to screen anti-idiotypic (anti-id) antibodies their ability stimulate anti-tumour T-cell responses. The human anti-id monoclonal (MAb) 105AD7 stimulated both delayed-type hypersensitivity (DTH) in animals and antigen-specific blastogenesis IL-2 induction advanced cancer patients. may not be necessary use as murine antibodies, which elicit DTH against immunodominant epitopes just useful the clinic. We have produced a same MAb was used generate screened it its immune Low-dose immunization with NCRC60, recognises paratope of anti-79ITgp72 79IT/36, induced 79ITgp72-expressing cells but antigen-negative cells. no detectable were 5 μg NCRC60 without adjuvant. Addition either complete Freund's adjuvant or Quil A did enhance However, when linked KLH injected presence anti-anti-id induced. also capable vitro priming T from patients proliferate response secondary stimulation cells, suggesting therapeutic potential © 1995 Wiley-Liss, Inc.