Genetic analysis and preliminary function study of miR-423 in breast cancer

摘要: Common genetic variants (single nucleotide polymorphisms SNPs) in microRNA (miRNA) genes may alter their maturation or expression and play a role the formation of human cancer. Recently, association between SNP rs6505162 pre-miR-423 cancer risk has been frequently evaluated diverse populations range cancers. In this study, we determined genotypes 5 matched cell lines (breast corresponding peripheral blood lines) 114 clinical specimens (clinical breast carcinoma normal tissues), compared processing efficiency pri-miRNA to mature forms pre-miR-423-12C (wild-type) pre-miR-423-12A (mutant-type) vectors, function miR-423 on proliferation. Our data showed that two out five 8.77 % (10/114) tumors underwent somatic mutations SNP, mutation state was significantly correlated with clinicopathologic variables, proliferating nuclear antigen (PCNA) mutant p53. The blocked endogenous pri-miR-423 its miRNAs. Interestingly, selected stable population had lower proliferation ability than population. Moreover, promoted through miR-423-3p strand, not miR-423-5p. Taken together, these results suggest affects miR expression, plays potentially oncogenic tumorigenesis.