Mediation of multiple pathways regulating cell proliferation, migration, and apoptosis in the human malignant glioma cell line U87MG via unphosphorylated STAT1: laboratory investigation.

摘要: Object Signal transducer and activator of transcription 1 (STAT1) is thought to be a tumor suppressor protein. The authors investigated the expression role STAT1 in glioblastoma. Methods Immunohistochemistry was used detect glioblastoma normal brain tissues. Reverse transcription–polymerase chain reaction Western blot analysis were mRNA protein levels STAT1. Cell growth, proliferation, migration, apoptosis, related genes proteins (Bcl-2, Bax, cleaved caspase-3, caspase-9, p21, proliferating cell nuclear antigen) examined vitro via counting kit-8, wound-healing, flow cytometry, Rhodamine B, TUNEL, assays. Results Human had decreased proteins. Transfection U87MG cells with plasmid demonstrated significant inhibition growth an increase apoptotic death compared transfected vector or m...