Angiogenesis involvement by octacalcium phosphate-gelatin composite-driven bone regeneration in rat calvaria critical-sized defect

摘要: Abstract Effect of octacalcium phosphate/gelatin composite (OCP/Gel) on angiogenesis was studied by its implantation in rat calvaria critical-sized defect relation to bone regeneration for 2 and 4 weeks. The OCP/Gel disks analyzed radiomorphometry using a radiopaque material perfusion (Microfil®) method histomorphometry hematoxylin eosin-staining before after the decalcification. OCP dose range up 4 mg per well capillary-like tube formation human umbilical vein endothelial cells (HUVECs) also examined transwell cell culture. results showed that blood vessels group significantly higher at 2 weeks than other groups but decreased 4 weeks during advancement new formation. highest an 1 mg while doses above or below did not show such stimulatory effect. established both vivo vitro confirmed has positive effect suitable ranges, suggesting stimulated could be involved OCP/Gel-enhanced regeneration. Statement significance We have reported phosphate (OCP) materials display capacities tissue-related cells. However, OCP-enhanced is unknown. This study capacity increasing mass gelatin (OCP/Gel). took place associated with area beginning formation, which finally together development Because ascertained stimulating HUVEC culture certain dose, present first report showing