Molecular detection of thyroglobulin mRNA transcripts in peripheral blood of patients with thyroid disease by RT-PCR

摘要: The sensitive detection of circulating tumour cells in patients with differentiated thyroid cancer may precede the relapse by other diagnostic studies – such as serum thyroglobulin and thus have important therapeutic prognostic implications. We performed reverse transcription-polymerase chain reaction (RT-PCR) on blood samples from diagnosed disease using two different RT-PCR sensitivities. Additionally, tissue specificity TG mRNA-expression was determined RNA extracts 27 human tissues. lower limit 50–100 mRNA producing cells/ml a ‘normal’ sensitivity 10–20 ‘high’ sensitivity. With normal detected 9/13 metastasis, 63/137 history no 21/85 non-malignant 9/50 controls. high 11/13 111/137 61/85 41/50 Interestingly, transcripts are specific for detectable peripheral controls disease, which correlates diagnosis metastasized cancer. However, sensitivity, expression found not to be correlated patients. As consequence, date cannot recommended marker superior serum-level. © 2000 Cancer Research Campaign