Interleukin-3-dependent expression of the c-myc and c-fos proto-oncogenes in hemopoietic cell lines.

摘要: Abstract Using Northern blot analysis the expression of several proto-oncogenes was studied in established lines mast cell precursors. Upon removal interleukin-3 (IL-3) from culture medium, factor-dependent cells stop dividing. During first 7 h, however, normal amounts total cellular mRNAs are maintained, and this is reflected unchanged levels transcripts, such as actin, c-Ha-ras c-fes. In contrast, within 1.5 h IL-3 removal, c-myc c-fos decrease drastically addition at that stage quickly induces back found actively growing cultures. factor-independent cells, which proliferate even absence IL-3, high transcripts maintained growth factor. arrested by serum starvation, 10% massive but not c-myc, proliferation restored. The data suggest play an important role mediating multiple effects on hemopoietic progenitor cells.