Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing of Dermatophilus congolensis.

摘要: This study aimed to evaluate molecular typing methods useful for standardization of strains in experimental work on dermatophilosis. Fifty Dermatophilus congolensis isolates, collected from sheep, cattle, horse and a deer, were analyzed by randomly amplified polymorphic DNA (RAPD) method using twenty-one different primers, the results compared with those obtained pulsed field gel electrophoresis (PFGE) restriction digest enzyme Sse8387I. The typeability, reproducibility discriminatory power RAPD Sse8387I-PFGE calculated. Both highly reproducible. Of two techniques, was least discriminating (Dice Index (DI), 0.663) could not distinguish between epidemiologically related whereas showed an excellent (DI, 0.7694–0.9722). Overall, degree correlation PFGE significantly high (r, 0.8822). We conclude that profiles generated either or can be used differentiate unrelated isolates. this strongly suggest at independent primers are order improve its power, is confirmation results.