The future is now: single-cell genomics of bacteria and archaea.

摘要: Interest in the expanding catalog of uncultivated microorganisms, increasing recognition heterogeneity among seemingly similar cells, and technological advances whole-genome amplification single-cell manipulation are driving considerable progress genomics. Here, spectrum applications for genomics, key development field, emerging methodology genome sequencing reviewed by example with attention to diversity approaches their unique characteristics. Experimental strategies transcending specific methodologies identified organized as a road map future studies genomics environmental microorganisms. Over next decade, increasingly powerful tools analysis will play roles accessing genomes organisms, determining basis microbial community functions, fundamental aspects population biology.