Lidocaine inhibits proliferation and induces apoptosis in colorectal cancer cells by upregulating mir-520a-3p and targeting EGFR

摘要: Abstract Lidocaine is a conventional local anesthetic which shown antiproliferative of colorectal cancer (CRC) in patients. MicroRNAs (miRNAs) have been consistently demonstrated to be involved CRC, and miR-520a-3p could suppress CRC migration, promote apoptosis by targeting epidermal growth factor receptor (EGFR). However, the mechanism lidocaine regulated proliferation remains unknown. In this study, quantitative RT-PCR were used measure EGFR expression levels, western blotting assays ware performed cells. Luciferase reporter assay was employed validate direct miR-520a-3p. Cell utilized analyze role The results indicated that 500 1000 μM over 24 h inhibited induced Compared with control group, suppressed (500 μM) Furthermore, directly targets increased rescued reduction caused inhibitor. suggested upregulating miR-520a-3p, it induce inhibit may serve as potential therapeutic regimen for cancer.