Dissociation between T helper type 1 and type 2 differentiation and cytokine production in tumor-infiltrating lymphocytes in patients with lung cancer.
作者： Norimasa Ito , Hiroshige Nakamura , Hiroyuki Metsugi , Shigetsugu Ohgi
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摘要: We recently reported the balance of T helper type 1 (Th1) cells and 2 (Th2) in patients with lung carcinomas. This study was conducted to investigate their activity role tumors, which remain unclear. determined population lymphocytes intracellular interferon (IFN)-γ or interleukin (IL)-4 by flow cytometry, investigated cytokine production using enzyme-linked immunosorbent assay (ELISA) 22 non-small cell cancer. The IFN-γ-positive subset showed a significant increase number tumor-infiltrating (TIL) compared peripheral blood (PBL) (PBL, 13.8% ± 1.5%; TIL, 34.3% 3.4%; P < 0.001), IL-4 positive reverse results 3.7% 0.6%; 2.1% 0.3%; = 0.037). However, TIL did not produce more IFN-γ than PBL. analysis PBL were significantly correlated (PBL: n 22, r 0.50, 0.025; TIL: 0.44, 0.022). dissociation between Th1 differentiation one host factors influencing immune anergy against tumors.
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