A lectin microarray approach for the rapid analysis of bacterial glycans.
作者： Ku-Lung Hsu , Lara K Mahal
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摘要: Rapid evaluation of microbial cell-surface carbohydrates is essential to understanding the mechanisms by which bacteria use glycans establish pathogenic or symbiotic relationships. Microbial glycan analysis complicated both vast diversity possible carbohydrate structures and their dynamic nature. Bacteria can rapidly alter coats switching genes that are involved on off in a phase-variable manner. Currently, there lack appropriate tools for studying alterations. Here, we present lectin microarray protocol high-throughput sugars. The binding patterns fluorescent these arrays provide simple means fingerprint based surface carbohydrates. In addition, this method provides rapid, parallel from multiple bacterial samples, allowing changes be studied. entire procedure takes approximately 12 h but printing performed advance.
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