Multiple dispersed spontaneous mutations: a novel pathway of mutation in a malignant human cell line.

摘要: The authors analyzed the nature of spontaneous mutations at autosomal locus coding for adenine phosphoribosyltransferase in human colorectal carcinoma cell line SW620 to establish whether distinctive mutational pathways exist that might underlie more complex genome rearrangements arising tumor cells. Point occur a low rate part hemizygotes derived from SW620, largely as result base substitutions G {center dot} C pairs yield transversions and transitions. However, novel pathway is evident form multiple dispersed which two errors, separated by much 1,800 bp, fall same mutant gene. Such could be error-prone DNA synthesis occurring during normal replication or long-patch excision-repair spontaneously lesions. This process also contribute chromosomal instability these