Multiple mutations of the p53 gene in human mammary carcinoma.

摘要: Alteration of the p53 tumor suppressor gene is most common genetic abnormality in human cancer. In breast cancer, depending on stage disease and method detection, mutation rates 25-60% have been observed. Multiple mutations same however, are rarely reported. this study we explored frequency multiple mammary carcinoma a cohort south Florida patients. Three hundred eighty-four cases primary cancer diagnosed between 1984 1986 at University Miami, Jackson Medical Center were subjects study. Sequence analysis exons 5 through 8 was performed cloned PCR-amplified DNA formalin-fixed, paraffin-embedded tumors. Two thirty-four 384 cancers (61%) had mutation. Of those, 36 tumors showed more than one mutation; 31 two mutations, three three, five case carried six mutations. The majority missense (43) followed by silent (35); occurred within single exon. Our suggests that currently believed.