MiR-335 functions as a tumor suppressor in pancreatic cancer by targeting OCT4.

作者: Ling Gao , Yijin Yang , Haiyan Xu , Ruqian Liu , Dechun Li

DOI: 10.1007/S13277-014-2092-9

关键词:

摘要: Octamer-binding transcription factor 4 (OCT4) was closely related to pancreatic cancer progression, but its regulation in by microRNA (miRNA) is not fully clear. OCT4-positive and OCT4-negative cells were isolated flow cytometry, it found that are enriched transplanted compared with the primary ones showed increasing proliferation sphere formation. The data of miRNA array assay miR-335 lower than negative ones. results confirmed tissue cell lines. Through expression analysis, underexpressed OCT4(+) purified from tumors. Enforced inhibited clonogenic expansion tumor development. re-expression blocked. Systemically delivered metastasis extended animal survival. Of significance, OCT4 identified validated as a direct functional target miR-335. Taken together, our provide evidence might inhibit progression stem properties targeting OCT4.

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