A novel porcine cell culture based protocol for the propagation of hepatitis E virus

摘要: Abstract Objective To present a comprehensive protocol for the processing of hepatitis E virus (HEV) infected samples and propagation in primary cell cultures. Methods Hepatitis was extracted from porcine liver faecal following standard protocols. The then allowed to attach presence trypsin cells that included bovine intestinal epithelial macrophages over period up 3 h. propagated by rotational passaging through Propagation confirmed immunoblotting. Results We developed propagate HEV model includes (i) culturing between types, (ii) pre-incubation 210 min, (iii) use semi-complete culture medium supplemented with (0.33 µg/mL) (iv) simple immunoblot technique detect amplified based on open reading frame 2/3. Conclusions This opens doors towards systematic analysis mechanisms underlie pathogenesis vitro . Using our protocol, one can complete process within 6 9 d.