Peripheral Distribution of Nervous System-Specific S-100 Prote'n in Rat

摘要: S-100 protein, a nervous system-specific was determined in soluble extract of various rat tissues with sensitive enzyme immunoassay method, which consisted solid-phase immobilized anti-S-100 antibody and the labeled beta-D-galactosidase from Escherichia coli. The minimum detectable amount protein 3 pg/assay. Central (cerebrum, cerebellum, brain stem) contained 1.4 to 2.8 micrograms protein/mg whereas most peripheral less than 0.05 microgram/ml specific protein. However, level high adipose tissue (0.5--1.1 micrograms/mg) trachea (about 0.5 microgram/mg), involves cartilage. levels several were significantly higher female rats males at ages 5 6 weeks.