Sialyltransferase activity in FR3T3 cells transformed with ras oncogene: decreased CMP-Neu5Ac:Galβ1-3GalNAc α-2,3-sialyltransferase
作者： P. Delannoy , H. Pelczar , V. Vandamme , A. Verbert
DOI: 10.1007/BF00731192
关键词:
摘要: We have investigated the activity of CMP-Neu5Ac:Gal\1-3GalNAc α-2,3-sialyltransferase (EC 2.4.99.4) in FR3T3 cells transformed by Ha-ras oncogene which we previously demonstrated higher expression β-galactosidase α-2,6-sialyltransferase 2.4.99.1) [21]. demonstrate that presence activatedras gene decreases this specific fourfold. According to kinetic parameters and mixing experiments, can assume decreased enzymatic reflects a decrease number activeO-glycan polypeptides inras-transformed cells. However, no change binding Peanut agglutinin was observed on cell surface ofras-transformed suggesting sialylation ofO-glycan core 1 appeared these cells, although decreased.
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