Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition.

摘要: We investigated whether the post-expansion redifferentiation and cartilage tissue formation capacity of adult human nasal chondrocytes can be regulated by controlled modifications scaffold composition architecture. As a model system, we used poly(ethylene glycol)-terephthalate–poly(butylene)-terephthalate block copolymer scaffolds from two compositions (low or high PEG content, resulting in different wettability) architectures (generated compression molding three-dimensional (3D) fiber deposition) with similar porosity mechanical properties, but interconnecting pore architectures. Scaffolds were seeded expanded constructs assessed immunohistochemically, biochemically at mRNA expression level following up to 4 weeks static culture. For given 3D architecture, more hydrophilic enhanced cell cartilaginous after culture, as higher collagen type II, increased deposition glycosaminoglycan (GAG) predominance II over I immunostain. The fiber-deposited scaffolds, accessible volume larger pores, supported GAG deposition, only if was used. By applying selective chemico-physical parameters, demonstrate that both architecture are instructive for generation tissues. observed effects likely have been mediated, respectively, differential serum protein adsorption efficiency nutrient/waste exchange.