Rem2 GTPase maintains survival of human embryonic stem cells as well as enhancing reprogramming by regulating p53 and cyclin D1.

摘要: Human pluripotent stem cells, such as embryonic cells (hESCs) and induced (iPSCs), have the unique abilities of differentiation into any cell type organism (pluripotency) indefinite selfrenewal. Here, we show that Rem2 GTPase, a suppressor p53 pathway, is up-regulated in hESCs and, by loss- gain-of-function studies, it major player maintenance hESC self-renewal pluripotency. We mediates fibroblastic growth factor 2 (FGF2) signaling pathway to maintain proliferation hESCs. demonstrate effects are mediated suppressing transcriptional activity cyclin D1 survival Importantly, does this preventing protein degradation during DNA damage. Given maintains hESCs, also efficient c-Myc enhancing reprogramming human somatic iPSCs eightfold. accelerating cycle protecting from apoptosis via its on expression/localization suppression transcription. independent function. These results define rate-limiting step phenomena. Our studies highlight possibility imposing hESC-specific features for making safer therapy use.