Amperometric immunosensor for diagnosis of BLV infection

作者: Bogumila Kurtinaitiene , Dovile Ambrozaite , Valdas Laurinavicius , Almira Ramanaviciene , Arunas Ramanavicius

DOI: 10.1016/J.BIOS.2008.01.014

关键词:

摘要: A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The antibody-antigen complex formation based on application secondary labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators this immunosensor. Optimal conditions found. Sensitivity created system compared the results enzyme-linked immunosorbent assay (ELISA) agar gel immunodiffusion (AGID) reaction, sufficient usual anti-gp51 antibody concentration present in blood serum BLV-infected cattle.

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