Purification and chemical characterization of α-fetoprotein from rat and mouse

摘要: Alpha-fetoprotein of the rat was immunochemically purified and characterized. Mouse α-fetoprotein, which cross-reacts with antibodies to also purified. Rat α-fetoprotein heterogeneous in disc electrophoresis isoelectric focusing had two different points at pH 4.76 5.05. homogeneous when analyzed similarly. The molecular weight both mouse 70,000 as determined by gel presence sodium dodecyl sulfate. specimens revealed a close similarity some other physicochemical, chemical immunological properties. Purification et Caracterisation Chimique de L'α-Fœtoproteine Souris L'α-fœtoproteine ete immunochimiquement purifiee caracterisee. L'α-fœtoproteine souris, qui des rections croisees avec les anticorps contre l'α-fœtoproteine rat, egalement purifiee. est heterogene, comme l'indiquent l'electrophorese en d'acrylamide le reperage du point isoelectrique, elle deux isoelectriques differents Les měmes analyses ont montre que souris homogene. Le poids moleculaire α-fœtoproteines 70 000, on l'a determine par electrophorese dodecyle sulfate sodium. On constate chez une etroite ressemblance vue quelques autres proprietes physicochimiques. chimiques immunologiques.