Diversity of virus–host systems in hypersaline Lake Retba, Senegal

摘要: Summary Remarkable morphological diversity of virus-like par- ticles was observed by transmission electron micros- copy in a hypersaline water sample from Lake Retba, Senegal. The majority particles morphologically resembled hyperthermophilic archaeal DNA viruses isolated extreme geothermal environments. Some viral morphotypes have not been previously nature, and less than 1% had head-and-tail morphology, which is typical for bacterial viruses. Culture- independent analysis the microbial suggested dominance extremely halophilic archaea. Few 16S sequences corre- sponded to known archeal genera (Haloquadratum, Halorubrum Natronomonas), whereas major- ity represented novel clades. Three corresponded new basal lineage haloarchaea. Bacteria belonged four major phyla, consistent with saline Metagenomic sequencing purified revealed very few similarities NCBI non-redundant database at either nucleotide or amino acid level. identifiable virus were most similar described haloarchaeal viruses, but no sequence found A large pro- portion similarity sequenced metagenomes solar salterns.