Comparison of the hydrophobic properties of Candida albicans and Candida dubliniensis.

作者: Kevin C. Hazen , Jean G. Wu , James Masuoka

DOI: 10.1128/IAI.69.2.779-786.2001

关键词:

摘要: Although Candida dubliniensis is a close genetic relative of albicans, it colonizes and infects fewer sites. Nearly all instances candidiasis caused by C. are restricted to the oral cavity. As cell surface hydrophobicity (CSH) influences virulence CSH properties were investigated compared albicans. Growth temperature one factor which affects status stationary-phase However, dubliniensis, similar other pathogenic non-albicans species Candida, was hydrophobic regardless growth temperature. For tested in this study (C. glabrata, krusei, parapsilosis, tropicalis), correlated with coaggregation anaerobic bacterium Fusobacterium nucleatum. Previous studies have shown that albicans involves multiple proteins protein N-glycans. The glycoprotein CAgp38 appears be expressed constitutively medium. expresses 38-kDa cross-reacts anti-CAgp38 monoclonal antibody; however, expression medium dependent. antibody has been inhibit adhesion extracellular matrix vascular endothelial cells. Since glycosylation we wall mannoprotein content composition between dubliniensis. Similar bulk compositional levels hexose, phosphate, their N-glycans determined. component N-glycan, acid-labile phosphooligomannoside, much less or negligibly when present, oligomannosides predominantly than five mannose residues length. In addition, phosphooligomannoside profiles varied among three strains tested, indicating N-glycan differs from fibrillar conformation, exposure proteins. Given combined role specific pathogenesis on proteins, lack these virulence-associated entities could contribute its limited ability cause disseminated infections.

参考文章(61)
Baqui Aa, Jabra-Rizk Ma, Brown Dm, Meiller Tf, Falkler Wa, Identification of Candida dubliniensis in a study of HIV-seropositive pediatric dental patients. Pediatric Dentistry. ,vol. 22, pp. 234- 238 ,(2000)
D Sullivan, D Bennett, M Henman, P Harwood, S Flint, F Mulcahy, D Shanley, D Coleman, Oligonucleotide fingerprinting of isolates of Candida species other than C. albicans and of atypical Candida species from human immunodeficiency virus-positive and AIDS patients. Journal of Clinical Microbiology. ,vol. 31, pp. 2124- 2133 ,(1993) , 10.1128/JCM.31.8.2124-2133.1993
H. L. Sheehan, J. C. Davis, Renal ischæmia with failed reflow The Journal of Pathology and Bacteriology. ,vol. 78, pp. 105- 120 ,(1959) , 10.1002/PATH.1700780112
Frank C. Odds, Luc Van Nuffel, Géry Dams, Prevalence of Candida dubliniensis Isolates in a Yeast Stock Collection Journal of Clinical Microbiology. ,vol. 36, pp. 2869- 2873 ,(1998) , 10.1128/JCM.36.10.2869-2873.1998
Mary Ann Jabra-Rizk, William A. Falkler, William G. Merz, Jacqueline I. Kelley, A. A. M. A. Baqui, Timothy F. Meiller, Coaggregation of Candida dubliniensis with Fusobacterium nucleatum. Journal of Clinical Microbiology. ,vol. 37, pp. 1464- 1468 ,(1999) , 10.1128/JCM.37.5.1464-1468.1999
S Mercure, S Sénéchal, P Auger, G Lemay, S Montplaisir, Candida albicans serotype analysis by flow cytometry. Journal of Clinical Microbiology. ,vol. 34, pp. 2106- 2112 ,(1996) , 10.1128/JCM.34.9.2106-2112.1996
P P Antley, K C Hazen, Role of yeast cell growth temperature on Candida albicans virulence in mice. Infection and Immunity. ,vol. 56, pp. 2884- 2890 ,(1988) , 10.1128/IAI.56.11.2884-2890.1988
K C Hazen, J G Lay, B W Hazen, R C Fu, S Murthy, Partial biochemical characterization of cell surface hydrophobicity and hydrophilicity of Candida albicans. Infection and Immunity. ,vol. 58, pp. 3469- 3476 ,(1990) , 10.1128/IAI.58.11.3469-3476.1990
W L Whelan, J M Delga, E Wadsworth, T J Walsh, K J Kwon-Chung, R Calderone, P N Lipke, Isolation and characterization of cell surface mutants of Candida albicans. Infection and Immunity. ,vol. 58, pp. 1552- 1557 ,(1990) , 10.1128/IAI.58.6.1552-1557.1990