作者: Kevin C. Hazen , Jean G. Wu , James Masuoka
DOI: 10.1128/IAI.69.2.779-786.2001
关键词:
摘要: Although Candida dubliniensis is a close genetic relative of albicans, it colonizes and infects fewer sites. Nearly all instances candidiasis caused by C. are restricted to the oral cavity. As cell surface hydrophobicity (CSH) influences virulence CSH properties were investigated compared albicans. Growth temperature one factor which affects status stationary-phase However, dubliniensis, similar other pathogenic non-albicans species Candida, was hydrophobic regardless growth temperature. For tested in this study (C. glabrata, krusei, parapsilosis, tropicalis), correlated with coaggregation anaerobic bacterium Fusobacterium nucleatum. Previous studies have shown that albicans involves multiple proteins protein N-glycans. The glycoprotein CAgp38 appears be expressed constitutively medium. expresses 38-kDa cross-reacts anti-CAgp38 monoclonal antibody; however, expression medium dependent. antibody has been inhibit adhesion extracellular matrix vascular endothelial cells. Since glycosylation we wall mannoprotein content composition between dubliniensis. Similar bulk compositional levels hexose, phosphate, their N-glycans determined. component N-glycan, acid-labile phosphooligomannoside, much less or negligibly when present, oligomannosides predominantly than five mannose residues length. In addition, phosphooligomannoside profiles varied among three strains tested, indicating N-glycan differs from fibrillar conformation, exposure proteins. Given combined role specific pathogenesis on proteins, lack these virulence-associated entities could contribute its limited ability cause disseminated infections.