A conformation change of single stranded polyriboadenyiate induced by an electric field

摘要: Abstract Absorbance measurements performed with high molecular weight poly A at pH 8 show that the degree of single strand stacking present ionic strength is reduced low strengths. The salt dependence conformation assigned to an electrostatic repulsion between subsequent turns “helix” structure. - Electric fields 5 80 kV/cm induce increase in absorbance consistent a decrease ion concentration environment polymer. linear function field suggesting change caused by dissociation effect. At increasing strength, threshold values electric have be exceeded order measurable changes. time required for decreases from about 2 μsec 10−4 M 0.3 strengths equilibration may influence rate limiting step, whereas arrangement nucleotide residues into ordered structure