Identification of factor inhibitors by diagnostic haemostasis laboratories. A large multi-centre evaluation

摘要: We have assessed the proficiency of diagnostic haemostasis facilities to correctly identify coagulation factor abnormalities and inhibitors. Forty-two laboratories participating in external Quality Assurance Program (QAP) conducted by RCPA agreed participate were each sent a set eight samples (each 3x1ml) for evaluation.They asked blind test these presence or absence inhibitors, where identified, perform further analysis (including specific inhibitor analysis). In order make exercise more challenging, addition true provided that reflected potential pre-analytical variables might arise complicate detection lead false identification. brief, sample comprised high level (F) V inhibitor, moderate FVIII (but was defibrinogenated), lupus anticoagulant (LA), normal slightly aged) plasma sample, serum EDTA an oral anticoagulant/vitamin K deficiency gross heparin (~10U/ml) contaminated sample. Sixty-three percent participants identified FV as such, although reported range varied greatly [10 >250 Bethesda units (BU/ml)] 46% despite complication presentation, also (7 64 BU/ml). Some either failed present, misidentified type.The LA, deficiency, sample,and (aged) most laboratories, inhibitors samples. However, small subset incorrectly some samples.The (68%) laboratories. contrast, and/or laboratories,and only one laboratory this sample.Thus, we conclude are able, cases, inhibitors,there is large variation levels there significant errors identification (i.e. negatives misidentifications). addition, positive error rate will no such exists positives).