Embryo rescue and plant regeneration following interspecific crosses in the genus Hylocereus (Cactaceae)

摘要: The aim of this study was to develop an efficient methodology rescue embryos following interspecific crosses in the genus Hylocereus. Crosses between diploids Hylocereus polyrhizus and H. undatus both directions were performed. Fertilized ovules carrying at very early pro-embryonic stages excised from ovaries 5 days after pollination (DAP) placed on half-strength basal MS medium containing 680 μM glutamine, 0.55 α-naphthaleneacetic acid (NAA), 0.45 thidiazuron (TDZ) various concentrations sucrose. After 30 culture, isolated surrounding tissue transferred same fresh medium. Significant differences found main effects (cross sucrose concentration) ovule response, i.e., increased size callus formation. best responses obtained cross: × undatus; concentration 0.09 M. In terms embryo conversion, polyembryony number regenerated plants, highest observed culture supplemented with 0.17 M crosses. All tested plants be diploid by flow cytometric analyses. Fluorescent amplified—fragment length polymorphism (fAFLP) confirmed hybrid origin plants. This reports success a three-step procedure for species. developed here provides means producing very-early stage, thus expanding prospects vine-cactus breeding programs.