Altered monoterpene composition in transgenic mint following the introduction of 4S-limonene synthase

摘要: Abstract Genetic transformation of Mentha arvensis and M.  ×  piperita with the neomycine phosphotransferase marker gene a 4 S -limonene synthase cDNA from spicata led to regeneration 47 transformed plants. In these plants, integration transgene was checked by PCR Southern blots. Transcripts were detected RT-PCR monoterpene profiles determined gas chromatography. Quantitative qualitative modifications in levels observed both transgenic Four plants contained higher total monoterpenes, whereas lower two lines. lines, altered compounds formed directly geranyl diphosphate such as cineole or ocimene end-products pulegone piperitone observed. These effects consistent either over-expression co-suppression 4S-ls gene, depending on line. data demonstrate feasibility modifying essential oil content introduction encoding an enzyme involved metabolism.