Application and Exploration of Fast Gas Chromatography - Surface Acoustic Wave Sensor to the Analysis of Thymus Species

作者: Se Yeon Oh , Jae Won Ko , Seo-Young Jeong , Jongki Hong

DOI: 10.1016/J.CHROMA.2008.07.078

关键词: Gas chromatographyChemotypeAromaSolid-phase microextractionGas chromatography–mass spectrometryChemical compositionThymolChemistryChromatographySample preparation

摘要: Abstract Fast gas chromatography combined with surface acoustic wave sensor (GC/SAW) has been applied for the detection of volatile aroma compounds emanated from thymus medicinal plants such as T. quinquecostotus (Jeju and Mt. Gaya in South Korea), var. japonica (Ulreung island mongolicus (Northeastern Asia), serpyllum (Europe). The GC/SAW involving fragrance pattern analysis provides a novel analytical method very fast separation characterization aromas caused by delicate difference chemical composition according to botanical geographical origin. On comparison experiments, characteristic components tendency air-dried species detected appear be quite similar those obtained headspace solid-phase microextraction (HS-SPME)-GC–MS, but abundance ratios between these two methods are different. In addition that, discrimination various using VaporPrint image based on reliable result. basis principal component (PCA) results, ability classification among completely different chemotypes HS-SPME-GC–MS is good enough, same which origin country, original its variety, an air-drying term 13 days 16 months much lower than GC/SAW. Interestingly, present experiment reveals that influences composition: concentration pharmacologically active species, monoterpene phenol (thymol), reaches highest concentrations after it was dried 5 or days, higher fresh over-dried long times.

参考文章(22)
Miroslav Kovačevič, Milica Kač, Solid-phase microextraction of hop volatiles Journal of Chromatography A. ,vol. 918, pp. 159- 167 ,(2001) , 10.1016/S0021-9673(01)00719-1
M.Consuelo Dı́az-Maroto, M.Soledad Pérez-Coello, M.Dolores Cabezudo, Supercritical carbon dioxide extraction of volatiles from spices ☆: Comparison with simultaneous distillation–extraction Journal of Chromatography A. ,vol. 947, pp. 23- 29 ,(2002) , 10.1016/S0021-9673(01)01585-0
A.J. Poulose, Rodney Croteau, Biosynthesis of aromatic monoterpenes Archives of Biochemistry and Biophysics. ,vol. 187, pp. 307- 314 ,(1978) , 10.1016/0003-9861(78)90039-5
Florence Diemer, Jean-Claude Caissard, Sandrine Moja, Jean-Claude Chalchat, Frédéric Jullien, Altered monoterpene composition in transgenic mint following the introduction of 4S-limonene synthase Plant Physiology and Biochemistry. ,vol. 39, pp. 603- 614 ,(2001) , 10.1016/S0981-9428(01)01273-6
Se Yeon Oh, Hyun Du Shin, Sung Jean Kim, Jongki Hong, Rapid determination of floral aroma compounds of lilac blossom by fast gas chromatography combined with surface acoustic wave sensor. Journal of Chromatography A. ,vol. 1183, pp. 170- 178 ,(2008) , 10.1016/J.CHROMA.2008.01.024
Michaela Zimmermann, P. Schieberle, Important odorants of sweet bell pepper powder (Capsicum annuum cv. annuum): differences between samples of Hungarian and Morrocan origin European Food Research and Technology. ,vol. 211, pp. 175- 180 ,(2000) , 10.1007/S002170050019
P H Gouyon, Ph Vernet, J L Guillerm, G Valdeyron, Polymorphisms and environment: the adaptive value of the oil polymorphisms in Thymus vulgaris L. Heredity. ,vol. 57, pp. 59- 66 ,(1986) , 10.1038/HDY.1986.87
M.J. Jordán, R.M. Martínez, K.L. Goodner, E.A. Baldwin, J.A. Sotomayor, Seasonal variation of Thymus hyemalis Lange and Spanish Thymus vulgaris L. essential oils composition Industrial Crops and Products. ,vol. 24, pp. 253- 263 ,(2006) , 10.1016/J.INDCROP.2006.06.011
Carlo Bicchi, Stefania Drigo, Patrizia Rubiolo, Influence of fibre coating in headspace solid-phase microextraction-gas chromatographic analysis of aromatic and medicinal plants. Journal of Chromatography A. ,vol. 892, pp. 469- 485 ,(2000) , 10.1016/S0021-9673(00)00231-4