Automated measurement of urinary creatinine by multichannel kinetic spectrophotometry.

摘要: Abstract Urinary creatinine analysis is required for clinical diagnosis, especially evaluation of renal function. Creatinine adjustment also widely used to estimate 24-h excretion from spot samples. Few convenient validated approaches are available in-house measurement small- medium-scale studies. Here we apply the Jaffe reaction determination with zone fluidic multichannel kinetic spectrophotometry. Diluted urine sample and reagent, alkaline picric acid, were mixed by a computer-programmed dispenser rapidly delivered four-channel detection cell. The absorbance change was monitored flow-through light-emitting diode–photodiode-based detector. Validation results against high-performance liquid chromatography–ultraviolet (HPLC–UV)/mass spectrometry (MS) presented. Responses 10-fold diluted samples linear within clinically relevant ranges (0–250 mg/L after dilution). system can analyze 70 per hour limit 0.76 mg/L. relative standard deviation 1.29% at 100 mg/L (n = 225). Correlation HPLC (UV quantitation/MS confirmation) excellent (linear, r2 = 0.9906). developed allows rapid, simple, cost-effective, robust suitable large numbers