Effect of glycosylation inhibitors on the production and properties of human leukocyte interferon.

摘要: Abstract Human leukocyte “buffy coat” suspensions were induced to produce interferon by stimulation with Sendai virus and incubated during the production period in medium containing various concentrations of glycosylation inhibitors, either d -glucosamine or 2-deoxy-D-glucose. At 80 mM at 8 2-deoxy- -glucose, was inhibited approximately 95%; however, 20 2 mM, respectively, these inhibitors allowed about 50% levels produced leukocytes not treated inhibitors. The residual interferons presence antigenically biologically indistinguishable from native human terms neutralization antisera antiviral activities on cells heterologous species. However, when analyzed electrophoresis SDS-polyacrylamide gels, exhibited markedly less size heterogeneity than found interferons, shifting toward smaller forms. Treatment 0.01 M sodium periodate buffer, pH 4.5 4° reduced lower molecular weight form; such treatment had no detectable effect