Serum Levels of 20-Kilodalton Human Growth Hormone (GH) Are Parallel Those of 22-Kilodalton Human GH in Normal and Short Children

作者: Mayumi Ishikawa , Susumu Yokoya , Katsuhiko Tachibana , Yukihiro Hasegawa , Toshiaki Yasuda

DOI: 10.1210/JCEM.84.1.5402

关键词:

摘要: Twenty-kilodalton human GH (20K), which is one of the (hGH) variants, thought to be produced by alternative premessenger ribonucleic acid splicing. However, its physiological role still unclear due lack a specific assay. We have measured serum 20K and 22-kDa hGH (22K) ELISAs investigate in children. The subjects were 162 normal children, aged 1 month 20 yr; 12 patients with deficiency (GHD), 11 months 13 57 children non-GHD short stature, 2-17 girls Turner's syndrome, 5 15 yr. Samples collected at random from after provocative tests 3-h nocturnal sleep GHD, syndrome mean basal concentrations 22K 2.4 +/- 2.8 ng/mL 152.3 184.0 pg/mL boys 2.5 3.1 130.6 171.5 girls, respectively. percentages (%20K) 5.8 2.1% 6.0 3.2% 83 79 There was no significant difference %20K either among ages or between prepubertal stage pubertal girls. values samples 6.5 2.4%, 3.8%, 5.9 3.2%, these growth disorders, there throughout disorders. also correlation percentage height SD score body mass index In conclusion, constant, regardless age, sex, puberty, score, index, secretion status. regulation levels remains established.

参考文章(25)
U.J. Lewis, J.T. Dunn, L.F. Bonewald, B.K. Seavey, W.P. Vanderlaan, A naturally occurring structural variant of human growth hormone. Journal of Biological Chemistry. ,vol. 253, pp. 2679- 2687 ,(1978) , 10.1016/S0021-9258(17)40874-X
J Smal, J Closset, G Hennen, P De Meyts, Receptor binding properties and insulin-like effects of human growth hormone and its 20 kDa-variant in rat adipocytes. Journal of Biological Chemistry. ,vol. 262, pp. 11071- 11079 ,(1987) , 10.1016/S0021-9258(18)60928-7
Frances M. DeNoto, David D. Moore, Howard M. Goodman, Human growth hormone DNA sequence and mRNA structure: possible alternative splicing Nucleic Acids Research. ,vol. 9, pp. 3719- 3730 ,(1981) , 10.1093/NAR/9.15.3719
James McCarter, Melissa A. Shaw, Lori A. Winer, Gerhard Baumann, The 20,000 Da variant of human growth hormone does not bind to growth hormone receptors in human liver. Molecular and Cellular Endocrinology. ,vol. 73, pp. 11- 14 ,(1990) , 10.1016/0303-7207(90)90039-B
GERHARD BAUMANN, MELISSA A. SHAW, Plasma transport of the 20,000-dalton variant of human growth hormone (20K): evidence for a 20K-specific binding site. The Journal of Clinical Endocrinology and Metabolism. ,vol. 71, pp. 1339- 1343 ,(1990) , 10.1210/JCEM-71-5-1339
Cesar L Boguszewski, Chatarina Jansson, Margaret CS Boguszewski, Sten Rosberg, Björn Carlsson, Kerstin Albertsson-Wikland, Lena MS Carlsson, None, Increased proportion of circulating non-22-kilodalton growth hormone isoforms in short children: a possible mechanism for growth failure. The Journal of Clinical Endocrinology and Metabolism. ,vol. 82, pp. 2944- 2949 ,(1997) , 10.1210/JCEM.82.9.4226
Sylvain Dor�, Guy R. Brisson, Alain Fournier, Richard Montpetit, H�l�ne Perrault, Dominique Boisvert, Contribution of hGH20K variant to blood hGH response in sauna and exercise. European Journal of Applied Physiology. ,vol. 62, pp. 130- 134 ,(1991) , 10.1007/BF00626768
U. J. Lewis, R. N. P. Singh, G. F. Tutwiler, Hyperglycemic Activity of the 20,000-Dalton Variant of Human Growth Hormone Endocrine Research Communications. ,vol. 8, pp. 155- 164 ,(1981) , 10.3109/07435808109045736
L.G. Frigeri, S.M. Peterson, U.J. Lewis, The 20,000-dalton structural variant of human growth hormone: lack of some early insulin-like effects. Biochemical and Biophysical Research Communications. ,vol. 91, pp. 778- 782 ,(1979) , 10.1016/0006-291X(79)91947-8