Altered effector functions of virus-specific and virus cross-reactive CD8+ T cells in mice immunized with related flaviviruses.

摘要: Memory cross-reactive CD8+ T-cell responses may induce protection or immunopathology upon secondary viral challenge. To elucidate the potential role of T cells in sequential flavivirus infection, we characterized CD4+ and between attenuated pathogenic Japanese encephalitis virus (JEV) West Nile (WNV). A previously reported WNV NS4b epitope its JEV variant elicited both JEV- WNV-infected mice. The peptide homologous to immunizing induced greater cytokine secretion activated higher frequencies epitope-specific cells. However, there was a virus-dependent, variant-independent pattern secretion; IFNγ+-to-IFNγ+TNFα+ ratio than Despite similarities burden for viruses, from JEV-immunized mice exhibited functional phenotypic profiles similar those seen strain. Patterns killer cell lectin-like receptor G1 (KLRG1) CD127 expression differed by type, with rapid expansion contraction short-lived effector infection persistence high levels infection. Such primary affect outcomes infections.