Effects of Schisandra chinensis (Wuweizi) constituents on the activity of hepatic microsomal CYP450 isozymes in rats detected by using a cocktail probe substrates method

摘要: Effects of constituents from Schisandra chinensis (Wuweizi) on six liver microsomal CYP450 isozymes (CYP1A2, CYP2C6, CYP2C11, CYP2D2, CYP2E1 and CYP3A1/2) were studied in rats vivo vitro. The vitro incubation was conducted using microsomes after multiple dosing alcoholic/water extract chinensis. A HPLC-MS method applied to determine the metabolites formation CYP450s probe substrates (phenacetin-CYP1A2, dextromethorphan-CYP2D2, diclofenac sodium-CYP2C6, mephenytoin-CYP2C11, chlorzoxazone-CYP2E1 midazolam-CYP3A1/2) rat incubations. activity represented by metabolites. Alcoholic (28-120 microg x mL(-1)) showed significant inhibitory effect a certain extent Multiple alcoholic (1.5 g kg(-1), qd 7d) had induction CYP3A1/2, inhibition CYP2D2 no CYP2C6 CYP1A2. Water (100-500 also exhibited vitro, whereas administrations CYP1A2 or CYP2C11. results suggested that possibility interaction between coadministrative drugs will be considered base levels subtype involved drug metabolism.