Influence of Surface Characteristics on the Stability of Cryptosporidium parvum Oocysts

摘要: Microelectrophoresis is a common technique for probing the surface chemistry of Cryptosporidium parvum oocyst. Results previous studies electrophoretic mobility C. oocysts in which microelectrophoresis was used are incongruent. In this work we demonstrated that capillary electrophoresis may also be to probe characteristics oocysts, and related their stability water. Capillary results indicated were washed phosphate buffer solution had neutrally charged surfaces. Inactivation with formalin did not influence mobility, while oocyst populations distilled water consisted cells both neutral negative charges. These indicate washing low-ionic-strength can impart charge fraction sample. Rapid coagulation experiments aggregate 0.5 M NaCl solution; salt have been result Lewis acid-base forces, steric stabilization, or some other factor. The presence sucrose Percoll could readily identified on by attenuated total reflectance-Fourier transform infrared spectroscopy, suggesting these purification reagents responsible uncharged oocysts. findings imply precipitate enmeshment optimal mechanism removal treatment systems. help elucidate causes variation characteristics, ultimately lead improved efficiencies full-scale systems, improve fate transport predictions natural