The differential regulation of formation of chemical and electrical connections inHelisoma

摘要: Novel chemical and electrical connections form between neurons not normally connected in the buccal ganglia of snail Helisoma. We examined cellular environmental conditions required for formation each type connection. Previous work situ showed that novel could response to axotomy. have now found axotomy can evoke unidirectional B5 B4 addition a The display all normal properties synapses Helisoma ganglia. These connections, however, are transient nature break 4 days following axotomy. Previous has shown conjoint outgrowth is connections. In cell culture we investigated whether also synaptogenesis. Using B19 when neuron pairs make contact culture, under synchronous neurite extension, both form. However, if one ceased extension prior by growing neuron, never (Hadley et al., 1983, 1985) but do Furthermore, serotonin (10−6M) medium inhibit B19, B5, selectively prevents while permitting synapses. Thus, timing relation state specify connection given