Gallic Acid an Agricultural Byproduct Modulates the Biofilm Matrix Exopolysaccharides of the Phytopathogen Ralstonia solanacearum

摘要: Ralstonia solanacearum is a soil-borne plant pathogen which causes wilt disease in economically important crops of the Solanaceae family tropical and temperate regions. As biofilm formation major virulence factor R. solanacearum, research inputs are necessary to identify natural inhibitors mitigate this bacterium. Hence present work, anti-biofilm potential phytochemical compound gallic acid (GA) isolated from an agricultural byproduct (cashewnut shell) was investigated. Initially Minimum inhibitory concentration (MIC) crude extracts cashewnut shell coconut against were The MIC both 400 µg/ml their sub-MIC (200 µg/ml) inhibited biofilms range 62–70% 49–57%, respectively. extract have higher effect compared extract, we proceeded our further study by isolating GA hydrolysate method. 85% young biofilms. observed at 3 mg/ml (1.5 mg/ml) found eradicate mature confirmed standard crystal violet assay reduction visualized under light microscopy scanning electron microscopic images. Toxicity evaluated through XTT cell viability no antibacterial sub-MIC. Additionally, it with growth curve time kill assays. Swimming twitching motility considered as factors invade plants block xylem vessels. Therefore, inhibit swimming about 93% 63% Anti-biofilm efficacy also worked well tomato model where remarkable inhibition on treatment before after 24 h infection solanacearum. will be alternative, cheap source eco-friendly novel for prevent crops.